catalog number :
MBS722344
products type :
ELISA Kit
products full name :
Rat Progranulin ELISA Kit
products short name :
Progranulin
other names :
progranulin; Progranulin; Progranulin; Uncharacterized protein
products gene name :
PRGN
other gene names :
LOC100692931
uniprot entry name :
C7E2T9_ORENI
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
products categories :
Neurobiology
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This PGRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PGRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY PGRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGRN antibody and an PGRN-HRP conjugate. The assay sample and buffer are incubated together with PGRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGRN concentration since PGRN from samples and PGRN-HRP conjugate compete for the anti-PGRN antibody binding site. Since the number of sites is limited, as more sites are occupied by PGRN from the sample, fewer sites are left to bind PGRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGRN concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ACT21831.1
ncbi mol weight :
22,499 Da