ELISA/assay

Human TGF beta receptor type 2 (TGFBR2) ELISA Kit

MBS7223133

48-Strip-Wells

470 USD

ELISA Kit

Human TGF beta receptor type 2 (TGFBR2) ELISA Kit

TGF beta receptor type 2 (TGFBR2)

Human TGF b receptor type 2 (TGFBR2) ELISA Kit

TGF-beta receptor type-2 isoform B; TGF-beta receptor type-2; TGF-beta receptor type-2; tbetaR-II; TGF-beta type II receptor; TGF-beta receptor type IIB; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II beta; transforming growth factor, beta receptor II alpha; transforming growth factor, beta receptor II delta; transforming growth factor, beta receptor II gamma; transforming growth factor, beta receptor II epsilon; transforming growth factor, beta receptor II (70/80kDa); TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II

TGFBR2

TGFBR2

TGFBR2; TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII; TGFR-2; TGF-beta receptor type II; TbetaR-II

TGFR2_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of TGF-betaR2. No significant cross-reactivity or interference between TGF-betaR2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TGF-betaR2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TGF-betaR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TGF-betaR2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

. Principle of the assay: TGF-betaR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGF-betaR2 antibody and an TGF-betaR2-HRP conjugate. The assay sample and buffer are incubated together with TGF-betaR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF-betaR2 concentration since TGF-betaR2 from samples and TGF-betaR2-HRP conjugate compete for the anti-TGF-betaR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF-betaR2 from the sample, fewer sites are left to bind TGF-betaR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF-betaR2 concentration in each sample is interpolated from this standard curve.

67782324

NP_003233.4

NM_003242.5

67,457 Da

ALK1 Signaling Events Pathway (137968); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Cytokine-cytokine Receptor Interaction Pathway (83051)

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

TGFBR2: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Binds to DAXX. Defects can cause esophageal cancer. Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); Kinase, protein; Oncoprotein; EC 2.7.11.30; Membrane protein, integral; TKL group; STKR family; Type2 subfamily. Chromosomal Location of Human Ortholog: 3p22. Cellular Component: plasma membrane; integral to membrane; caveola; cytosol; receptor complex; external side of plasma membrane. Molecular Function: transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; transforming growth factor beta receptor activity, type II; transforming growth factor beta binding; metal ion binding; mitogen-activated protein kinase kinase kinase binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity. Biological Process: lens development in camera-type eye; wound healing; apoptosis; positive regulation of smooth muscle cell proliferation; heart development; myeloid dendritic cell differentiation; gastrulation; palate development; positive regulation of tolerance induction to self antigen; protein amino acid phosphorylation; negative regulation of cardiac muscle cell proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to glucose stimulus; positive regulation of NK T cell differentiation; vasculogenesis; positive regulation of T cell tolerance induction; response to nutrient; aging; response to drug; receptor-mediated endocytosis; blood vessel development; organ regeneration; smoothened signaling pathway; positive regulation of skeletal muscle regeneration; in utero embryonic development; embryonic hemopoiesis; common-partner SMAD protein phosphorylation; peptidyl-threonine phosphorylation; embryonic cranial skeleton morphogenesis; regulation of cell proliferation; patterning of blood vessels; peptidyl-serine phosphorylation; positive regulation of angiogenesis; gut development; regulation of gene expression; response to mechanical stimulus; response to estrogen stimulus; positive regulation of B cell tolerance induction; activation of protein kinase activity; brain development; negative regulation of transforming growth factor beta receptor signaling pathway; embryo implantation. Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 6; Loeys-dietz Syndrome 2; Esophageal Cancer

48-Strip-Wells

470 USD

96-Strip-Wells

675