ELISA/assay

Rat Stromal Cell Derived Factor 1 ELISA Kit

MBS722311

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Stromal Cell Derived Factor 1 ELISA Kit

Stromal Cell Derived Factor 1

stromal cell-derived factor 1; stromal cell-derived factor 1; chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); chemokine (C-X-C motif) ligand 12

SDF1

CXCL12; SDF1

Rat

This assay has high sensitivity and excellent specificity for detection of SDF-1. No significant cross-reactivity or interference between SDF-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDF-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This SDF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay SDF-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDF-1. Standards or samples are then added to the microtiter plate wells and SDF-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDF-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDF-1 are added to each well to "sandwich" the SDF-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDF-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1 concentration in each sample is interpolated from this standard curve.

45383151

NP_989841.1

NM_204510.1

Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Intestinal Immune Network For IgA Production Pathway (128762); Intestinal Immune Network For IgA Production Pathway (128670)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675