ELISA/assay

Mouse PaRathyroid Hormone Related Protein ELISA Kit

MBS722308

48-Strip-Wells

470 USD

ELISA Kit

Mouse PaRathyroid Hormone Related Protein ELISA Kit

PaRathyroid Hormone Related Protein

parathyroid hormone-related protein preproprotein; Parathyroid hormone-related protein; parathyroid hormone-related protein; PTHrP; PTH-rP; parathyroid-like peptide; parathyroid hormone-like peptide; parathyroid hormone-like protein; parathyroid hormone-like hormone; Parathyroid hormone-like protein; PLP

PTHrP

Pthlh; Pthlh; PLP; Pthrp; PTH-rP; PTHrP; PLP

PTHR_RAT

Mouse

This assay has high sensitivity and excellent specificity for detection of PTHrP. No significant cross-reactivity or interference between PTHrP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTHrP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This PTHrP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PTHrP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: PTHrP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTHrP antibody and an PTHrP-HRP conjugate. The assay sample and buffer are incubated together with PTHrP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTHrP concentration since PTHrP from samples and PTHrP-HRP conjugate compete for the anti-PTHrP antibody binding site. Since the number of sites is limited, as more sites are occupied by PTHrP from the sample, fewer sites are left to bind PTHrP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTHrP concentration in each sample is interpolated from this standard curve.

6981438

NP_036768.1

NM_012636.1

20,204 Da

Endochondral Ossification Pathway (219771)

induces vasodilation; may play a neuroprotective role in response to ischemic brain injury [RGD, Feb 2006]

PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described. Protein type: Secreted, signal peptide; Secreted. Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; extracellular region; intracellular; nucleus. Molecular Function: peptide hormone receptor binding; hormone activity. Biological Process: negative regulation of transcription factor activity; negative regulation of chondrocyte differentiation; G-protein signaling, adenylate cyclase activating pathway; bone mineralization; osteoblast development; epithelial cell differentiation; regulation of gene expression; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; protein processing; regulation of chondrocyte differentiation; alveolus development; surfactant homeostasis; skeletal development; endochondral ossification; endoderm development

48-Strip-Wells

470 USD

96-Strip-Wells

675