Mouse Apolipoprotein C2 ELISA Kit | MyBioSource MBS722303 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Apolipoprotein C2 ELISA Kit

catalog :

MBS722303

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS722303

products type :

ELISA Kit

products full name :

Mouse Apolipoprotein C2 ELISA Kit

products short name :

Apolipoprotein C2

other names :

apolipoprotein C-II; Apolipoprotein C-II; apolipoprotein C-II; apolipoprotein C2; apolipoprotein C-II; Apolipoprotein C2

products gene name :

APOC2

other gene names :

APOC2; APOC2; APO-CII; APOC-II; APC2; Apo-CII; ApoC-II

uniprot entry name :

APOC2_HUMAN

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This Apo-C2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Apo-C2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the Assay: Apo-C2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Apo-C2. Standards or samples are then added to the microtiter plate wells and Apo-C2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Apo-C2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for Apo-C2 are added to each well to "sandwich" the Apo-C2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Apo-C2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-C2 concentration in each sample is interpolated from this standard curve. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

ncbi gi num :

32130518

ncbi acc num :

NP_000474.2

ncbi gb acc num :

NM_000483.4

ncbi mol weight :

11,284 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Retinoid Metabolism And Transport Pathway (187208); Signal Transduction Pathway (477114)

ncbi summary :

This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. This gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally occurring read-through transcription exists between this gene and the neighboring upstream apolipoprotein C-IV (APOC4) gene. [provided by RefSeq, Mar 2011]

uniprot summary :

APOC2: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly. Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B). It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Belongs to the apolipoprotein C2 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: extracellular space; chylomicron; early endosome; extracellular region. Molecular Function: protein homodimerization activity; phospholipase binding; lipase inhibitor activity; lipid binding; phospholipase activator activity. Biological Process: positive regulation of fatty acid biosynthetic process; phototransduction, visible light; negative regulation of cholesterol transport; lipoprotein metabolic process; cholesterol efflux; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; negative regulation of catalytic activity; positive regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; retinoid metabolic process; negative regulation of lipid metabolic process; lipid catabolic process. Disease: Apolipoprotein C-ii Deficiency

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage