ELISA/assay

Human Platelet Activating Factor Acetylhydrolase ELISA Kit

MBS722279

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Platelet Activating Factor Acetylhydrolase ELISA Kit

Platelet Activating Factor Acetylhydrolase

platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; group VII phospholipase A2; lipoprotein-associated phospholipase A2

PAF AH

PLA2G7; PAF-AH; Lp-PLA2

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This PAF-AH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PAF-AH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: PAF-AH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAF-AH antibody and an PAF-AH-HRP conjugate. The assay sample and buffer are incubated together with PAF-AH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAF-AH concentration since PAF-AH from samples and PAF-AH-HRP conjugate compete for the anti-PAF-AH antibody binding site. Since the number of sites is limited, as more sites are occupied by PAF-AH from the sample, fewer sites are left to bind PAF-AH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAF-AH concentration in each sample is interpolated from this standard curve.

163915175

NP_001106484.1

NM_001113013.1

Ether Lipid Metabolism Pathway (84416); Ether Lipid Metabolism Pathway (365); Metabolic Pathways (132936); Metabolism Of Proteins Pathway (1014364); Peptide Hormone Metabolism Pathway (1014412); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (1014414)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675