ELISA/assay

Rabbit H-ras ELISA Kit

MBS722274

96 Strip Wells

675 USD

ELISA Kit

Rabbit H-ras ELISA Kit

H-ras

H-ras, partial; GTPase NRas; GTPase NRas; neuroblastoma ras oncogene; transforming protein N-Ras; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; Transforming protein N-Ras

H-ras

NRAS; NRAS; HRAS; H-RAS

RASN_PIG

Rabbit

This assay has high sensitivity and excellent specificity for detection of H-ras. No significant cross-reactivity or interference between H-ras and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between H-ras and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This H-ras ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit H-ras. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Principle of the assay: H-ras ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-H-ras antibody and an H-ras-HRP conjugate. The assay sample and buffer are incubated together with H-ras-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the H-ras concentration since H-ras from samples and H-ras-HRP conjugate compete for the anti-H-ras antibody binding site. Since the number of sites is limited, as more sites are occupied by H-ras from the sample, fewer sites are left to bind H-ras-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H-ras concentration in each sample is interpolated from this standard curve.

6650730

AAF21987.1

21,229 Da

ARMS-mediated Activation Pathway 1013279!!Activation Of RAS In B Cells Pathway 1014254!!Acute Myeloid Leukemia Pathway 84524!!Acute Myeloid Leukemia Pathway 529!!Adaptive Immune System Pathway 1014238!!Alcoholism Pathway 585587!!Alcoholism Pathway 587116!!Axon Guidance Pathway 84477!!Axon Guidance Pathway 476!!Axon Guidance Pathway 1014044

96 Strip Wells

675 USD