ELISA/assay

Human P selectin ELISA Kit

MBS722270

96 Strip Wells

675 USD

ELISA Kit

Human P selectin ELISA Kit

P selectin

P-selectin; P-selectin; P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; selectin P (granule membrane protein 140kDa, antigen CD62); CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P

P selectin

SELP; SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM; GMRP; GRMP; GMP-140; LECAM3; PADGEM

LYAM3_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This P SELECTIN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human P SELECTIN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Immunology

Principle of the Assay: P SELECTIN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for P SELECTIN. Standards or samples are then added to the microtiter plate wells and P SELECTIN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of P SELECTIN present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for P SELECTIN are added to each well to "sandwich" the P SELECTIN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain P SELECTIN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P SELECTIN concentration in each sample is interpolated from this standard curve.

157419154

NP_002996.2

NM_003005.3

90,834 Da

Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!IL-3 Signaling Pathway 198881!!IL4-mediated Signaling Events Pathway 137933!!Malaria Pathway 152665!!Malaria Pathway 152657!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD