ELISA/assay

Rabbit Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit

MBS722261

48-Strip-Wells

440 USD

ELISA Kit

Rabbit Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit

[Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)]

[Rabbit Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit; Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF); Colony Stimulating Factor 2, Granulocyte Macrophage ELISA Kit (GMCSF) (Rabbit)]

[granulocyte-macrophage colony-stimulating factor]

[GMCSF]

Rabbit

This assay has high sensitivity and excellent specificity for detection of GMCSF. No significant cross-reactivity or interference between GMCSF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GMCSF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Rabbit ELISA Kit

Intended Uses: This GMCSF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GMCSF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GMCSF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GMCSF antibody and an GMCSF-HRP conjugate. The assay sample and buffer are incubated together with GMCSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GMCSF concentration since GMCSF from samples and GMCSF-HRP conjugate compete for the anti-GMCSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GMCSF from the sample, fewer sites are left to bind GMCSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GMCSF concentration in each sample is interpolated from this standard curve.

237690354

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48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415