ELISA/assay

Porcine CREB-regulated transcription coactivator 3 (CRTC3) ELISA Kit

MBS7222605

48-Strip-Wells

470 USD

ELISA Kit

Porcine CREB-regulated transcription coactivator 3 (CRTC3) ELISA Kit

CREB-regulated transcription coactivator 3 (CRTC3)

CREB-regulated transcription coactivator 3 isoform a; CREB-regulated transcription coactivator 3; CREB-regulated transcription coactivator 3; transducer of regulated CREB protein 3; transducer of regulated cAMP response element-binding protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3; CREB regulated transcription coactivator 3; Transducer of regulated cAMP response element-binding protein 3; TORC-3; Transducer of CREB protein 3

CRTC3

CRTC3; CRTC3; TORC3; TORC-3; TORC3; TORC-3; Transducer of CREB protein 3

CRTC3_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of CRTC3. No significant cross-reactivity or interference between CRTC3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRTC3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CRTC3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CRTC3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: CRTC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRTC3 antibody and an CRTC3-HRP conjugate. The assay sample and buffer are incubated together with CRTC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRTC3 concentration since CRTC3 from samples and CRTC3-HRP conjugate compete for the anti-CRTC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRTC3 from the sample, fewer sites are left to bind CRTC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRTC3 concentration in each sample is interpolated from this standard curve.

110431354

NP_073606.3

NM_022769.4

66,830 Da

HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

TORC3: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 Ser-133 phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter. Arg-314 in the bZIP domain of CREB1 is essential for this interaction. Interaction with HTLV-1 TAX enhances its transcriptional activity. Interacts, via the N- terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription. Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach. Belongs to the TORC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 15q26.1. Cellular Component: nucleoplasm; cytoplasm. Molecular Function: cAMP response element binding protein binding. Biological Process: viral reproduction; transcription, DNA-dependent; negative regulation of lipid catabolic process; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; protein homotetramerization

48-Strip-Wells

470 USD

96-Strip-Wells

675