ELISA/assay

Rabbit Acid Sphingomyelinase ELISA Kit

MBS722256

96 Strip Wells

675 USD

ELISA Kit

Rabbit Acid Sphingomyelinase ELISA Kit

Acid Sphingomyelinase

acid sphingomyelinase; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase; acid sphingomyelinase; sphingomyelin phosphodiesterase 1, acid lysosomal; Acid sphingomyelinase; aSMase

ASM

SMPD1; SMPD1; ASM; NPD; ASMASE; ASM; aSMase

ASM_HUMAN

Rabbit

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cell Biology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This SDC1/CD138 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDC1/CD138. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY SDC1/CD138 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDC1/CD138. Standards or samples are then added to the microtiter plate wells and SDC1/CD138 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDC1/CD138 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDC1/CD138 are added to each well to “sandwich” the SDC1/CD138 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDC1/CD138 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC1/CD138 concentration in each sample is interpolated from this standard curve.

179095

AAA58377.1

69,624 Da

Ceramide Signaling Pathway 138023!!FAS (CD95) Signaling Pathway 138081!!Glycosphingolipid Metabolism Pathway 530751!!IL2 Signaling Events Mediated By PI3K Pathway 137938!!Lysosome Pathway 99052!!Lysosome Pathway 96865!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Ovarian Infertility Genes Pathway 198801

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

96 Strip Wells

675 USD