ELISA/assay

Rabbit Cross-linked Carboxy-terminal telopeptide of type I collagen ELISA Kit

MBS722244

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Cross-linked Carboxy-terminal telopeptide of type I collagen ELISA Kit

Cross-linked Carboxy-terminal telopeptide of type I collagen

Rabbit Cross-linked Carboxy-terminal telopeptide of type I collagen ELISA Kit; Cross-linked Carboxy-terminal telopeptide of type I collagen; Cross-linked Carboxy-terminal telopeptide of type I collagen (Rabbit)

ICTP

Rabbit

Sensitivity: The sensitivity in this assay is 0.1 ng/ml. Specificity: This assay has high sensitivity and excellent specificity for detection of ICTP. No significant cross-reactivity or interference between ICTP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Rabbit ELISA Kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This ICTP ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ICTP in the sample, this ICTP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ICTP concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ICTP utilizes a multiclonal anti-ICTP antibody and an ICTP-HRP conjugate. The assay sample and buffer are incubated together with ICTP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ICTP concentration since ICTP from samples and ICTP-HRP conjugate compete for the anti-ICTP antibody binding site. Since the number of sites is limited, as more sites are occupied by ICTP from the sample, fewer sites are left to bind ICTP-HRP conjugate. Standards of known ICTP concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ICTP. The ICTP concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675