ELISA/assay

Human Metabotropic glutamate receptor 1 (GRM1) ELISA Kit

MBS7222434

48-Strip-Wells

470 USD

ELISA Kit

Human Metabotropic glutamate receptor 1 (GRM1) ELISA Kit

Metabotropic glutamate receptor 1 (GRM1)

metabotropic glutamate receptor 1 isoform alpha; Metabotropic glutamate receptor 1; metabotropic glutamate receptor 1; protein phosphatase 1, regulatory subunit 85; glutamate receptor, metabotropic 1

GRM1

GRM1; GRM1; MGLU1; GPRC1A; MGLUR1; SCAR13; PPP1R85; GPRC1A; MGLUR1; mGluR1

GRM1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GRM1. No significant cross-reactivity or interference between GRM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRM1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GRM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRM1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: GRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRM1 antibody and an GRM1-HRP conjugate. The assay sample and buffer are incubated together with GRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRM1 concentration since GRM1 from samples and GRM1-HRP conjugate compete for the anti-GRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRM1 from the sample, fewer sites are left to bind GRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRM1 concentration in each sample is interpolated from this standard curve.

493798972

NP_001264993.1

NM_001278064.1

101,473 Da

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (106381); Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); FoxO Signaling Pathway (921162); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198839)

This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

mGluR1: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 3. Chromosomal Location of Human Ortholog: 6q24. Cellular Component: integral to plasma membrane; dendrite; postsynaptic density; plasma membrane; nucleus. Molecular Function: G-protein coupled receptor activity; protein binding; glutamate receptor activity. Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; activation of MAPK activity; protein kinase C activation; metabotropic glutamate receptor signaling pathway; locomotory behavior; regulation of sensory perception of pain; sensory perception of pain. Disease: Spinocerebellar Ataxia, Autosomal Recessive 13

48-Strip-Wells

470 USD

96-Strip-Wells

675