ELISA/assay

Mouse Glutaredoxin-1 (GLRX) ELISA Kit

MBS7222215

48-Strip-Wells

470 USD

ELISA Kit

Mouse Glutaredoxin-1 (GLRX) ELISA Kit

Glutaredoxin-1 (GLRX)

glutaredoxin-1; Glutaredoxin-1; glutaredoxin-1; TTase-1; thioltransferase-1; glutaredoxin (thioltransferase); Thioltransferase-1; TTase-1

GLRX

GLRX; GLRX; GRX; GRX1; GRX; TTase-1

GLRX1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of GLRX. No significant cross-reactivity or interference between GLRX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLRX and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This GLRX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GLRX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

. Principle of the assay: GLRX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLRX antibody and an GLRX-HRP conjugate. The assay sample and buffer are incubated together with GLRX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLRX concentration since GLRX from samples and GLRX-HRP conjugate compete for the anti-GLRX antibody binding site. Since the number of sites is limited, as more sites are occupied by GLRX from the sample, fewer sites are left to bind GLRX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLRX concentration in each sample is interpolated from this standard curve.

169646776

NP_001112362.1

NM_001118890.1

11,776 Da

Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway (161057); Ascorbate Recycling (cytosolic) Pathway (545345); Ascorbate Recycling (cytosolic) Pathway (545621)

This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]

GLRX1: Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Belongs to the glutaredoxin family. Protein type: Oxidoreductase. Chromosomal Location of Human Ortholog: 5q14. Cellular Component: mitochondrion; cytosol; nucleus. Molecular Function: electron carrier activity; protein N-terminus binding; glutathione disulfide oxidoreductase activity. Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; cell redox homeostasis; positive regulation of membrane potential

48-Strip-Wells

470 USD

96-Strip-Wells

675