Human Defensin 1, Neutrophil ELISA Kit | MyBioSource MBS722210 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Defensin 1, Neutrophil ELISA Kit

catalog :

MBS722210

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS722210

products type :

ELISA Kit

products full name :

Human Defensin 1, Neutrophil ELISA Kit

products short name :

Defensin 1, Neutrophil

other names :

defensin, alpha 1, myeloid-related sequence; Neutrophil defensin 1; neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence; defensin, alpha 1; Defensin, alpha 1; HNP-1; HP-1; HP1

products gene name :

DEFA1

other gene names :

DEFA1; DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1; DEF1; DEFA2; MRS DEFA1B ; HP-1; HP1; HP-2; HP2

uniprot entry name :

DEF1_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

products categories :

Immunology

products description :

Intended Uses: This DEF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DEF1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: DEF1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DEF1. Standards or samples are then added to the microtiter plate wells and DEF1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DEF1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DEF1 are added to each well to "sandwich" the DEF1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DEF1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEF1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

50057842

ncbi acc num :

AAT68878.1

ncbi mol weight :

10,201 Da

ncbi pathways :

Alpha-defensins Pathway (530758); Cellular Roles Of Anthrax Toxin Pathway (138076); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

uniprot summary :

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region. Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to virus; defense response to fungus

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage