ELISA/assay

Human Sodium/potassium transporting ATPase subunit beta 1 (ATP1B1) ELISA Kit

MBS7222044

48-Strip-Wells

470 USD

ELISA Kit

Human Sodium/potassium transporting ATPase subunit beta 1 (ATP1B1) ELISA Kit

Sodium/potassium transporting ATPase subunit beta 1 (ATP1B1)

Human Sodium/potassium transporting ATPase subunit b 1 (ATP1B1) ELISA Kit

sodium/potassium-transporting ATPase subunit beta-1; Sodium/potassium-transporting ATPase subunit beta-1; sodium/potassium-transporting ATPase subunit beta-1; adenosinetriphosphatase; sodium pump subunit beta-1; Na, K-ATPase beta-1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase beta-1 chain; sodium-potassium ATPase subunit beta 1 (non-catalytic); ATPase, Na+/K+ transporting, beta 1 polypeptide; Sodium/potassium-dependent ATPase subunit beta-1

ATP1B1

ATP1B1

ATP1B1; ATP1B1; ATP1B; ATP1B

AT1B1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ATP1beta1. No significant cross-reactivity or interference between ATP1beta1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP1beta1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This ATP1beta1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP1beta1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neuroscience

Principle of the assay: ATP1beta1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP1beta1 antibody and an ATP1beta1-HRP conjugate. The assay sample and buffer are incubated together with ATP1beta1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP1beta1 concentration since ATP1beta1 from samples and ATP1beta1-HRP conjugate compete for the anti-ATP1beta1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP1beta1 from the sample, fewer sites are left to bind ATP1beta1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP1beta1 concentration in each sample is interpolated from this standard curve.

4502277

NP_001668.1

NM_001677.3

34,893 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Basigin Interactions Pathway (106065); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Calcium Regulation In The Cardiac Cell Pathway (198906); Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654)

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]

ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q24. Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; vesicle; sarcolemma. Molecular Function: ATPase activator activity; protein binding; sodium:potassium-exchanging ATPase activity; ATPase binding. Biological Process: cellular sodium ion homeostasis; protein stabilization; cellular potassium ion homeostasis; cellular calcium ion homeostasis; potassium ion import; regulation of gene expression; positive regulation of ATPase activity; response to hypoxia; blood coagulation; cell adhesion; transmembrane transport; leukocyte migration; cardiac muscle contraction. Disease: Hypertension, Essential

48-Strip-Wells

470 USD

96-Strip-Wells

675