Human Galectin 3 ELISA Kit | MyBioSource MBS722196 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Galectin 3 ELISA Kit

catalog :

MBS722196

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS722196

products type :

ELISA Kit

products full name :

Human Galectin 3 ELISA Kit

products short name :

Galectin 3

other names :

galectin-3 isoform 2; Galectin-3; galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen

products gene name :

GAL3

other gene names :

LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP

uniprot entry name :

LEG3_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of GAL-3. No significant cross-reactivity or interference between GAL-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAL-3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GAL-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GAL-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the assay: GAL-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAL-3 antibody and an GAL-3-HRP conjugate. The assay sample and buffer are incubated together with GAL-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL-3 concentration since GAL-3 from samples and GAL-3-HRP conjugate compete for the anti-GAL-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL-3 from the sample, fewer sites are left to bind GAL-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL-3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

294345475

ncbi acc num :

NP_001170859.1

ncbi gb acc num :

NM_001177388.1

ncbi mol weight :

26,152 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

uniprot summary :

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages. Protein type: Extracellular matrix; Cell surface; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 14q22.3. Cellular Component: spliceosome; extracellular matrix; proteinaceous extracellular matrix; extracellular space; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane. Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity. Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; negative regulation of endocytosis; RNA splicing; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; epithelial cell differentiation; positive chemotaxis; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage