ELISA/assay

Mouse Growth Differentiation Factor 3 ELISA Kit

MBS722177

48-Strip-Wells

470 USD

ELISA Kit

Mouse Growth Differentiation Factor 3 ELISA Kit

Growth Differentiation Factor 3

growth/differentiation factor 3; Growth/differentiation factor 3; growth/differentiation factor 3; GDF-3; growth differentiation factor 3

GDF3

GDF3; GDF3; KFS3; MCOP7; MCOPCB6; UNQ222/PRO248; GDF-3

GDF3_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of GDF-3. No significant cross-reactivity or interference between GDF-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF-3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This GDF-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GDF-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: GDF-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF-3 antibody and an GDF-3-HRP conjugate. The assay sample and buffer are incubated together with GDF-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF-3 concentration since GDF-3 from samples and GDF-3-HRP conjugate compete for the anti-GDF-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF-3 from the sample, fewer sites are left to bind GDF-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-3 concentration in each sample is interpolated from this standard curve.

10190670

NP_065685.1

NM_020634.1

41,387 Da

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. [provided by RefSeq, Jul 2008]

GDF3: Defects in GDF3 are the cause of Klippel-Feil syndrome type 3 (KFS3); also called Klippel-Feil syndrome autosomal dominant 3. KFS3 is a skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Defects in GDF3 are the cause of microphthalmia isolated with coloboma type 6 (MCOPCB6); also called isolated colobomatous microphthalmia 6. MCOPCB6 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Defects in GDF3 are a cause of microphthalmia isolated type 7 (MCOP7). MCOP7 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 12p13.1. Cellular Component: extracellular space; cytoplasm. Molecular Function: growth factor activity; cytokine activity; protein kinase binding; transforming growth factor beta receptor binding. Biological Process: response to dietary excess; in utero embryonic development; somite rostral/caudal axis specification; notochord development; formation of anatomical boundary; negative regulation of BMP signaling pathway; regulation of apoptosis; eye development; regulation of MAPKKK cascade; negative regulation of epidermal cell differentiation; regulation of cell fate commitment; mesoderm development; negative regulation of myoblast differentiation; skeletal development; cell development; growth; endoderm development. Disease: Microphthalmia, Isolated 7; Klippel-feil Syndrome 3, Autosomal Dominant; Microphthalmia, Isolated, With Coloboma 6

48-Strip-Wells

470 USD

96-Strip-Wells

675