ELISA/assay

Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit

MBS722176

48-Strip-Wells

470 USD

ELISA Kit

Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit

Inhibitor of apoptosis protein Like Protein2

IAP-like protein 2; Baculoviral IAP repeat-containing protein 8; baculoviral IAP repeat-containing protein 8; IAP-like protein 2; baculoviral IAP repeat-containing 8; inhibitor of apoptosis-like protein 2; testis-specific inhibitor of apoptosis; baculoviral IAP repeat containing 8; Inhibitor of apoptosis-like protein 2; IAP-like protein 2; ILP-2; Testis-specific inhibitor of apoptosis

ILP2

BIRC8; BIRC8; ILP2; ILP-2; hILP2; ILP2; IAP-like protein 2; ILP-2

BIRC8_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of IPLP-2. No significant cross-reactivity or interference between IPLP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IPLP-2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This IPLP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IPLP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Priciple of the assay: IPLP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IPLP-2 antibody and an IPLP-2-HRP conjugate. The assay sample and buffer are incubated together with IPLP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IPLP-2 concentration since IPLP-2 from samples and IPLP-2-HRP conjugate compete for the anti-IPLP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IPLP-2 from the sample, fewer sites are left to bind IPLP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IPLP-2 concentration in each sample is interpolated from this standard curve.

15042064

AAK81892.1

27,089 Da

Apoptosis Pathway (83060); Apoptosis Pathway (470); Pathways In Cancer (83105); Small Cell Lung Cancer Pathway (83118); Small Cell Lung Cancer Pathway (530); Toxoplasmosis Pathway (169642); Toxoplasmosis Pathway (169493); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

BIRC8: Protects against apoptosis mediated by BAX. Belongs to the IAP family. Protein type: Apoptosis; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: -. Cellular Component: spindle microtubule; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protease binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity. Biological Process: neuron apoptosis; protein ubiquitination; negative regulation of neuron apoptosis; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675