Monkey Epoxide hydrolase 1 (EPHX1) ELISA Kit | MyBioSource MBS7221727 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Epoxide hydrolase 1 (EPHX1) ELISA Kit

catalog :

MBS7221727

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7221727

products type :

ELISA Kit

products full name :

Monkey Epoxide hydrolase 1 (EPHX1) ELISA Kit

products short name :

[Epoxide hydrolase 1 (EPHX1)]

other names :

[epoxide hydrolase 1; Epoxide hydrolase 1; epoxide hydrolase 1; epoxide hydratase; epoxide hydrolase 1, microsomal (xenobiotic); Epoxide hydratase; Microsomal epoxide hydrolase]

products gene name :

[EPHX1]

other gene names :

[EPHX1; EPHX1; MEH; EPHX; EPOX; HYL1; EPHX; EPOX]

uniprot entry name :

HYEP_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of EPB41L5. No significant cross-reactivity or interference between EPB41L5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EPB41L5 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

products description :

Intended Uses: This EPB41L5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse EPB41L5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: EPB41L5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPB41L5 antibody and an EPB41L5-HRP conjugate. The assay sample and buffer are incubated together with EPB41L5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPB41L5 concentration since EPB41L5 from samples and EPB41L5-HRP conjugate compete for the anti-EPB41L5 antibody binding site. Since the number of sites is limited, as more sites are occupied by EPB41L5 from the sample, fewer sites are left to bind EPB41L5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPB41L5 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

209862837

ncbi acc num :

NP_001129490.1

ncbi gb acc num :

NM_001136018.3

ncbi mol weight :

52,949 Da

ncbi pathways :

Aflatoxin B1 Metabolism Pathway (198808); Benzo(a)pyrene Metabolism Pathway (198911); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (425); Metapathway Biotransformation (198837)

ncbi summary :

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]

uniprot summary :

Ephx1: Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls. Defects in EPHX1 are a cause of familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the peptidase S33 family. Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; EC 3.3.2.9; Membrane protein, integral; Hydrolase. Chromosomal Location of Human Ortholog: 1q42.1. Cellular Component: endoplasmic reticulum membrane; integral to membrane. Molecular Function: cis-stilbene-oxide hydrolase activity; epoxide hydrolase activity. Biological Process: response to toxin; response to organic cyclic substance; aromatic compound catabolic process. Disease: Hypercholanemia, Familial; Preeclampsia/eclampsia 1

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage