ELISA/assay

Rat antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

MBS722100

48-Strip-Wells

470 USD

ELISA Kit

Rat antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

diuretic hormone/vasopressin/arginine vasopressin

Rat antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit; antidiuretic hormone/vasopressin/arginine vasopressin; antidiuretic hormone/vasopressin/arginine vasopressin (Rat)

Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; arginine vasopressin; AVP-NPIICleaved into the following 3 chains:Arg-vasopressin; Alternative name(s):; Arginine-vasopressinNeurophysin 2; Alternative name(s):; Neurophysin-II

ADH/VP/AVP

AVP; AVP; VP; ADH; ARVP; AVRP; AVP-NPII; ARVP; VP

NEU2_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ADH. No significant cross-reactivity or interference between ADH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Rat ELISA Kit

Intended Uses: This ADH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ADH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ADH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADH. Standards or samples are then added to the microtiter plate wells and ADH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADH are added to each well to "sandwich" the ADH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH concentration in each sample is interpolated from this standard curve.

116497129

AAI26197.1

P01185

17,325 Da

Aquaporin-mediated Transport Pathway (187187); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358)

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Function: Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Subcellular location: Secreted. Involvement in disease: Diabetes insipidus, neurohypophyseal (NDI) [MIM:125700]: A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopressin deficiency during childhood.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36. Sequence similarities: Belongs to the vasopressin/oxytocin family.

48-Strip-Wells

470 USD

96-Strip-Wells

675