Human Bisphosphoglycee mutase (BPGM) ELISA Kit | MyBioSource MBS7220891 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Bisphosphoglycee mutase (BPGM) ELISA Kit

catalog :

MBS7220891

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7220891

products type :

ELISA Kit

products full name :

Human Bisphosphoglycee mutase (BPGM) ELISA Kit

products short name :

Bisphosphoglycee mutase (BPGM)

other names :

bisphosphoglycerate mutase; Bisphosphoglycerate mutase; bisphosphoglycerate mutase; BPG-dependent PGAM; 2,3-diphosphoglycerate mutase; 2,3-bisphosphoglycerate synthase; erythrocyte 2,3-bisphosphoglycerate mutase; 2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate mutase; 2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase (EC:3.1.3.13, EC:5.4.2.11); 2,3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM

products gene name :

BPGM

other gene names :

BPGM; BPGM; DPGM; BPGM; DPGM

uniprot entry name :

PMGE_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of BPGM. No significant cross-reactivity or interference between BPGM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BPGM and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This BPGM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BPGM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: BPGM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BPGM antibody and an BPGM-HRP conjugate. The assay sample and buffer are incubated together with BPGM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPGM concentration since BPGM from samples and BPGM-HRP conjugate compete for the anti-BPGM antibody binding site. Since the number of sites is limited, as more sites are occupied by BPGM from the sample, fewer sites are left to bind BPGM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPGM concentration in each sample is interpolated from this standard curve.

ncbi gi num :

641386940

ncbi acc num :

NP_001280014.1

ncbi gb acc num :

NM_001293085.1

ncbi mol weight :

30,005 Da

ncbi pathways :

Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Metabolic Pathways (132956); Rapoport-Luebering Glycolytic Shunt Pathway (545320); Rapoport-Luebering Glycolytic Shunt Pathway (545483); Gluconeogenesis I Pathway (142180); Gluconeogenesis III Pathway (908097); Glycolysis I Pathway (142455)

ncbi summary :

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]

uniprot summary :

BPGM: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD). A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 5.4.2.11; EC 3.1.3.13; Isomerase; EC 5.4.2.4; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 7q33. Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity. Biological Process: erythrocyte development; glycolysis; dephosphorylation; carbohydrate metabolic process; respiratory gaseous exchange. Disease: Bisphosphoglycerate Mutase Deficiency

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage