ELISA/assay

Rat A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4 ELISA Kit

MBS722089

96 Strip Wells

675 USD

ELISA Kit

Rat A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4 ELISA Kit

A Disintegrin like and Metalloproteinase with Thrombospondin type 1 motif 4

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADMP-1; Aggrecanase-1

ADAMTS4

ADAMTS4; ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688; UNQ769/PRO1563; ADAM-TS 4; ADAM-TS4; ADAMTS-4

ATS4_HUMAN

Rat

Store all reagents at 2-8 degree C.

Cardiovascular

Intended Uses: This ADAMTS4 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofADAMTS4 in the sample, thisADAMTS4 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusADAMTS4 concentration. The concentration ofADAMTS4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This ADAMTS4 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forADAMTS4. Standards or samples are then added to the microtiter plate wells andADAMTS4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofADAMTS4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forADAMTS4 are added to each well to "sandwich" theADAMTS4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containADAMTS4 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

157427675

NP_005090.3

NM_005099.4

90,197 Da

Degradation Of The Extracellular Matrix Pathway 576263!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Metabolism Of Proteins Pathway 106230!!O-glycosylation Of TSR Domain-containing Proteins Pathway 980460!!O-linked Glycosylation Pathway 980459!!Post-translational Protein Modification Pathway 161012

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD