ELISA/assay

Human Maspin (serpin B5) ELISA Kit

MBS722088

48-Strip-Wells

470 USD

ELISA Kit

Human Maspin (serpin B5) ELISA Kit

Maspin (serpin B5)

maspin; Serpin B5; serpin B5; PI-5; peptidase inhibitor 5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; Maspin; Peptidase inhibitor 5; PI-5

Maspin

SERPINB5; SERPINB5; PI5; maspin; PI5; PI-5

SPB5_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of MSB5. No significant cross-reactivity or interference between MSB5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MSB5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This MSB5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MSB5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: MSB5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MSB5 antibody and an MSB5-HRP conjugate. The assay sample and buffer are incubated together with MSB5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSB5 concentration since MSB5 from samples and MSB5-HRP conjugate compete for the anti-MSB5 antibody binding site. Since the number of sites is limited, as more sites are occupied by MSB5 from the sample, fewer sites are left to bind MSB5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSB5 concentration in each sample is interpolated from this standard curve.

453369

AAA18957.1

25,763 Da

ATF-2 Transcription Factor Network Pathway (138006); Direct P53 Effectors Pathway (137939); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); P53 Signaling Pathway (83055); P53 Signaling Pathway (465)

maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 18q21.33. Cellular Component: extracellular space; cytoplasm. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding. Biological Process: extracellular matrix organization and biogenesis; morphogenesis of an epithelium; cell motility; regulation of epithelial cell proliferation

48-Strip-Wells

470 USD

96-Strip-Wells

675