ELISA/assay

Mouse Thymus Activation Regulated Chemokine ELISA Kit

MBS722054

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Mouse Thymus Activation Regulated Chemokine ELISA Kit

Thymus Activation Regulated Chemokine

thymus and activation-regulated chemokine; CC chemokine ABCD-2; chemokine (C-C motif) ligand 17; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A17; chemokine (C-C motif) ligand 17; CC chemokine ABCD-2; Chemokine (C-C motif) ligand 17; Thymus and activation-regulated chemokine

TARC

Ccl17; Ccl17; Tarc; Abcd-2; Scya17; Scya17l; ABCD-2; TARC; mCG_12286

Q9WUZ6_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of TARC. No significant cross-reactivity or interference between TARC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TARC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 1.0pg/mL.

Immunology

Intended Uses This TARC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TARC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TARC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TARC. Standards or samples are then added to the microtiter plate wells and TARC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TARC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TARC are added to each well to "sandwich” the TARC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TARC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TARC concentration in each sample is interpolated from this standard curve.

5102778

CAB45256.1

Q9WUZ6

10,467 Da

Chemokine Receptors Bind Chemokines Pathway (1000944); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (1000941); Peptide Ligand-binding Receptors Pathway (1000943); Signal Transduction Pathway (970540)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675