catalog number :
MBS722051
products type :
ELISA Kit
products full name :
Mouse Complement Component 2 ELISA Kit
products short name :
Complement Component 2
other names :
Complement component 2, isoform CRA_b; Complement component 2, isoform CRA_b; cDNA, FLJ92956, Homo sapiens complement component 2 (C2), mRNA
other gene names :
C2; hCG_43694
uniprot entry name :
Q5JP69_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of C2. No significant cross-reactivity or interference between C2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C2 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This C2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C2. This ELISA kit for research use only, not for therapeutic or diagnostic applications
products categories :
Immunology
products description :
Principle of the assay: C2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C2 antibody and an C2-HRP conjugate. The assay sample and buffer are incubated together with C2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C2 concentration since C2 from samples and C2-HRP conjugate compete for the anti-C2 antibody binding site. Since the number of sites is limited, as more sites are occupied by C2 from the sample, fewer sites are left to bind C2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C2 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAQ07481.1
ncbi mol weight :
83,268 Da