ELISA/assay

Rat Creatine Kinase ELISA Kit

MBS722021

48-Strip-Wells

470 USD

ELISA Kit

Rat Creatine Kinase ELISA Kit

Creatine Kinase

Rat Creatine Kinase (CK) ELISA Kit; Rat Creatine Kinase ELISA Kit; Creatine Kinase; Creatine Kinase (Rat)

creatine kinase; Creatine kinase U-type, mitochondrial; creatine kinase U-type, mitochondrial; U-MtCK; mia-CK; OTTHUMP00000066275; OTTHUMP00000200125; ubiquitous mitochondrial creatine kinase; acidic-type mitochondrial creatine kinase; creatine kinase, mitochondrial 1 (ubiquitous); creatine kinase, mitochondrial 1B; Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase

CK

CKMT1B; CKMT1A; CKMT; CKMT1; UMTCK; CKMT1A; CKMT

KCRU_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CK. No significant cross-reactivity or interference between CK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This CK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Rat ELISA Kit

Principle of the assay: CK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CK. Standards or samples are then added to the microtiter plate wells and CK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CK are added to each well to "sandwich" the CK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK concentration in each sample is interpolated from this standard curve.

180590

P12532

47,037 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Metabolic Pathways (132956); Metabolism Of Amino Acids And Derivatives Pathway (106169); Creatine-phosphate Biosynthesis Pathway (138632)

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Catalytic activity: ATP + creatine = ADP + phosphocreatine. Subunit structure: Exists as an octamer composed of four MTCK homodimers. Ref.9. Subcellular location: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Miscellaneous: Mitochondrial creatine kinase binds cardiolipin. Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

48-Strip-Wells

470 USD

96-Strip-Wells

675