Mouse Semaphorin 3 ELISA Kit | MyBioSource MBS722009 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Semaphorin 3 ELISA Kit

catalog :

MBS722009

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS722009

products type :

ELISA Kit

products full name :

Mouse Semaphorin 3 ELISA Kit

products short name :

Semaphorin 3

other names :

semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III

products gene name :

SEMA3

other gene names :

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

uniprot entry name :

SEM3A_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of SEMA3. No significant cross-reactivity or interference between SEMA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEMA3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This SEMA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SEMA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: SEMA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEMA3 antibody and an SEMA3-HRP conjugate. The assay sample and buffer are incubated together with SEMA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEMA3 concentration since SEMA3 from samples and SEMA3-HRP conjugate compete for the anti-SEMA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by SEMA3 from the sample, fewer sites are left to bind SEMA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5174673

ncbi acc num :

NP_006071.1

ncbi gb acc num :

NM_006080.2

ncbi mol weight :

88,889 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7p12.1. Cellular Component: membrane; axon; dendrite; extracellular region. Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding. Biological Process: trigeminal nerve structural organization; axon guidance; facial nerve structural organization; regulation of heart rate; apoptosis; dendrite morphogenesis; olfactory bulb development; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development. Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage