ELISA/assay

Mouse Selenoprotein P1, Plasma ELISA Kit

MBS721982

48-Strip-Wells

470 USD

ELISA Kit

Mouse Selenoprotein P1, Plasma ELISA Kit

Selenoprotein P1, Plasma

Selenoprotein P; Selenoprotein P; selenoprotein P; seP; selenoprotein P, plasma, 1

SEPP1

Sepp1; Sepp1; Selp; SeP

SEPP1_RAT

Mouse

This assay has high sensitivity and excellent specificity for detection of SEPP-1. No significant cross-reactivity or interference between SEPP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEPP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This SEPP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SEPP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

. Principle of the assay: SEPP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEPP-1 antibody and an SEPP-1-HRP conjugate. The assay sample and buffer are incubated together with SEPP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEPP-1 concentration since SEPP-1 from samples and SEPP-1-HRP conjugate compete for the anti-SEPP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SEPP-1 from the sample, fewer sites are left to bind SEPP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEPP-1 concentration in each sample is interpolated from this standard curve.

172046809

P25236.2

43,083 Da

Cardiovascular Signaling Pathway (219769); Integrin-mediated Cell Adhesion Pathway (198487); Selenium Pathway (219762); Selenium Metabolism Selenoproteins Pathway (219789)

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is a secreted protein, and is unusual in that it contains 10 selenocysteine residues per polypeptide, constituting most of the selenium in plasma. It has been implicated as an extracellular antioxidant and in the transport of selenium. Transcript variants differing in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: selenium binding. Biological Process: response to selenium ion; sexual reproduction; locomotory behavior; brain development; selenium metabolic process; post-embryonic development; growth

48-Strip-Wells

470 USD

96-Strip-Wells

675