Human Vimentin ELISA Kit | MyBioSource MBS721933 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Vimentin ELISA Kit

catalog :

MBS721933

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS721933

products type :

ELISA Kit

products full name :

Human Vimentin ELISA Kit

products short name :

Vimentin

other names :

vimentin; Vimentin; vimentin; epididymis luminal protein 113; vimentin

products gene name :

VIM

other gene names :

VIM; VIM; HEL113; CTRCT30

uniprot entry name :

VIME_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of VIM. No significant cross-reactivity or interference between VIM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VIM and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This VIM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VIM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: VIM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VIM antibody and an VIM-HRP conjugate. The assay sample and buffer are incubated together with VIM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VIM concentration since VIM from samples and VIM-HRP conjugate compete for the anti-VIM antibody binding site. Since the number of sites is limited, as more sites are occupied by VIM from the sample, fewer sites are left to bind VIM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VIM concentration in each sample is interpolated from this standard curve.

ncbi gi num :

62414289

ncbi acc num :

NP_003371.2

ncbi gb acc num :

NM_003380.3

ncbi mol weight :

53,652 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

ncbi summary :

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

uniprot summary :

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 10p13. Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol. Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens. Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; muscle filament sliding; cell structure disassembly during apoptosis. Disease: Cataract 30

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage