ELISA/assay

Guinea pig Cardiac Troponin T ELISA Kit

MBS721923

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Cardiac Troponin T ELISA Kit

Cardiac Troponin T

cardiac troponin T; Troponin T, cardiac muscle; troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant); troponin T type 2 (cardiac); Cardiac muscle troponin T; cTnT

cTnT

TNNT2; TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6; TnTc; cTnT

TNNT2_HUMAN

Guinea Pig

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich

Intended Uses: This cTnT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig cTnT. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This cTnT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig cTnT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: cTnT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for cTnT. Standards or samples are then added to the microtiter plate wells and cTnT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of cTnT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for cTnT are added to each well to "sandwich" the cTnT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain cTnT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnT concentration in each sample is interpolated from this standard curve.

15290516

AAK92231.1

30,681 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Motor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament. Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; actin binding; tropomyosin binding. Biological Process: atrial cardiac muscle morphogenesis; metabolic process; positive regulation of ATPase activity; regulation of muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding. Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2

48-Strip-Wells

470 USD

96-Strip-Wells

675