ELISA/assay

Rat Plasminogen activator inhibitor ELISA Kit

MBS721904

48-Strip-Wells

470 USD

ELISA Kit

Rat Plasminogen activator inhibitor ELISA Kit

Plasminogen activator inhibitor

plasminogen activator inhibitor; Plasminogen activator inhibitor 2; plasminogen activator inhibitor 2; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor

PAI

SERPINB2; SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201; PAI2; PLANH2; PAI-2

PAI2_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This PAI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PAI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: PAI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAI. Standards or samples are then added to the microtiter plate wells and PAI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAI present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PAI are added to each well to "sandwich" the PAI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAI concentration in each sample is interpolated from this standard curve.

189545

AAA36413.1

46,596 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Blood Clotting Cascade Pathway (198840); Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Senescence And Autophagy Pathway (198780)

SERPINB2: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell- derived PAI-1. Belongs to the serpin family. Ov-serpin subfamily. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: fibrinolysis; blood coagulation; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675