catalog number :
MBS721901
products type :
ELISA Kit
products full name :
Mouse Fibrinogen ELISA Kit
products short name :
Fibrinogen
other names :
fibrinogen; Fibrinogen gamma-B chain; fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide; fibrinogen gamma chain<
other gene names :
FGG; FGG; Gamma'
uniprot entry name :
FIBG_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of FbG. No significant cross-reactivity or interference between FbG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FbG and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This FbG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FbG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the assay: FbG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FbG. Standards or samples are then added to the microtiter plate wells and FbG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FbG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FbG are added to each well to “sandwich” the FbG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FbG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FbG concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAA33562.1
ncbi mol weight :
50,244 Da
ncbi pathways :
Common Pathway (1009072); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Extracellular Matrix Organization Pathway (1008838); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1009069); GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway (1008640); Hemostasis Pathway (1009046); Integrin AlphaIIb Beta3 Signaling Pathway (1008639); Integrin Cell Surface Interactions Pathway (1008854); Platelet Aggregation (Plug Formation) Pathway (1009064)
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)