ELISA/assay

Human Hyaluronan and proteoglycan link protein 1 (HAPLN1) ELISA Kit

MBS7218191

48-Strip-Wells

440 USD

ELISA Kit

Human Hyaluronan and proteoglycan link protein 1 (HAPLN1) ELISA Kit

[Hyaluronan and proteoglycan link protein 1 (HAPLN1)]

[hyaluronan and proteoglycan link protein 1; Hyaluronan and proteoglycan link protein 1; hyaluronan and proteoglycan link protein 1; Cartilage link protein; cartilage-link protein; cartilage linking protein 1; cartilage-linking protein 1; hyaluronan and proteoglycan link protein 1; Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link protein]

[HAPLN1]

[HAPLN1; HAPLN1; CRTL1; CRTL1; Cartilage-link protein]

HPLN1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of HA. No significant cross-reactivity or interference between HA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This HA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HA. Standards or samples are then added to the microtiter plate wells and HA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HA are added to each well to "sandwich" the HA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HA concentration in each sample is interpolated from this standard curve.

4503053

NP_001875.1

NM_001884.3

40,166 Da

ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262)

HAPLN1: Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Belongs to the HAPLN family. Protein type: Extracellular matrix. Chromosomal Location of Human Ortholog: 5q14.3. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region. Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding. Biological Process: extracellular matrix organization and biogenesis; central nervous system development; cell adhesion; skeletal development

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415