ELISA/assay

Rat G protein-coupled receptor 40 ELISA Kit

MBS721810

48-Strip-Wells

470 USD

ELISA Kit

Rat G protein-coupled receptor 40 ELISA Kit

G protein-coupled receptor 40

GPR40; Free fatty acid receptor 1; free fatty acid receptor 1; G protein-coupled receptor 40; G-protein coupled receptor 40; G protein-coupled receptor GPR40; free fatty acid receptor 1; G-protein coupled receptor 40

GPR40

Ffar1; Ffar1; Gpr40; Gpr40

FFAR1_MOUSE

Rat

This assay has high sensitivity and excellent specificity for detection of GPR-40. No significant cross-reactivity or interference between GPR-40 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPR-40 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GPR-40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GPR-40. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: GPR-40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPR-40 antibody and an GPR-40-HRP conjugate. The assay sample and buffer are incubated together with GPR-40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPR-40 concentration since GPR-40 from samples and GPR-40-HRP conjugate compete for the anti-GPR-40 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPR-40 from the sample, fewer sites are left to bind GPR-40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPR-40 concentration in each sample is interpolated from this standard curve.

22597182

AAN03478.1

31,804 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Fatty Acids Bound To GPR40 (FFAR1) Regulate Insulin Secretion Pathway (1001110); Free Fatty Acid Receptors Pathway (1000966); Free Fatty Acids Regulate Insulin Secretion Pathway (1001109); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); Gastrin-CREB Signalling Pathway Via PKC And MAPK (970815); Incretin Synthesis, Secretion, And Inactivation Pathway (1000417); Insulin Secretion Pathway (777543)

FFAR1: Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G- proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1. Cellular Component: membrane; integral to plasma membrane; cell; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; lipid binding; fatty acid binding; bioactive lipid receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; insulin secretion; positive regulation of insulin secretion; signal transduction; positive regulation of calcium ion transport; glucose homeostasis

48-Strip-Wells

470 USD

96-Strip-Wells

675