ELISA/assay

Rat Engrailed Homeobox Protein 2 ELISA Kit

MBS721798

48-Strip-Wells

470 USD

ELISA Kit

Rat Engrailed Homeobox Protein 2 ELISA Kit

Engrailed Homeobox Protein 2

Engrailed homeobox 2; Homeobox protein engrailed-2; homeobox protein engrailed-2; hu-En-2; engrailed homolog 2; homeobox protein en-2; engrailed homeobox 2

EN2

EN2; EN2; Homeobox protein en-2; Hu-En-2

HME2_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of EHP2. No significant cross-reactivity or interference between EHP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EHP2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This EHP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EHP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: EHP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EHP2 antibody and an EHP2-HRP conjugate. The assay sample and buffer are incubated together with EHP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EHP2 concentration since EHP2 from samples and EHP2-HRP conjugate compete for the anti-EHP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by EHP2 from the sample, fewer sites are left to bind EHP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EHP2 concentration in each sample is interpolated from this standard curve.

85397847

AAI04971.1

34,211 Da

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

En-2: Genetic variations in EN2 may be associated with susceptibility to autism. Belongs to the engrailed homeobox family. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 7q36. Cellular Component: membrane; nucleus. Molecular Function: sequence-specific DNA binding. Biological Process: multicellular organismal development; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; hindbrain development. Disease: Autism, Susceptibility To, 10

48-Strip-Wells

470 USD

96-Strip-Wells

675