ELISA/assay

Human Lanosterol 14 Alpha demethylase (CYP51A1) ELISA Kit

MBS7217723

48-Strip-Wells

470 USD

ELISA Kit

Human Lanosterol 14 Alpha demethylase (CYP51A1) ELISA Kit

Lanosterol 14 Alpha demethylase (CYP51A1)

lanosterol 14-alpha demethylase isoform 2; Lanosterol 14-alpha demethylase; lanosterol 14-alpha demethylase; CYPLI; cytochrome P450LI; cytochrome P45014DM; cytochrome P450 51A1; cytochrome P450-14DM; lanosterol 14-alpha-demethylase; cytochrome P450, 51 (lanosterol 14-alpha-demethylase); cytochrome P450, family 51, subfamily A, polypeptide 1; CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase

CYP51A1

CYP51A1; CYP51A1; LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM; CYP51; LDM; Cytochrome P45014DM

CP51A_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CYP51A1. No significant cross-reactivity or interference between CYP51A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP51A1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This CYP51A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYP51A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CYP51A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CYP51A1 antibody and an CYP51A1-HRP conjugate. The assay sample and buffer are incubated together with CYP51A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP51A1 concentration since CYP51A1 from samples and CYP51A1-HRP conjugate compete for the anti-CYP51A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP51A1 from the sample, fewer sites are left to bind CYP51A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP51A1 concentration in each sample is interpolated from this standard curve.

225903399

NP_001139624.1

NM_001146152.1

46,312 Da

Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); Biological Oxidations Pathway (105698); Cholesterol Biosynthesis Pathway (198809); Cholesterol Biosynthesis Pathway (106142); Cholesterol Biosynthesis, Squalene 2,3-epoxide = Cholesterol Pathway (413390); Cholesterol Biosynthesis, Squalene 2,3-epoxide = Cholesterol Pathway (468282); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Endogenous Sterols Pathway (105701); Metabolic Pathways (132956); Metabolism Pathway (477135)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CYP51A1: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4 -dimethyl cholesta-8,14,24-triene-3-beta-ol. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 1.14.13.70; Lipid Metabolism - steroid biosynthesis; Oxidoreductase. Chromosomal Location of Human Ortholog: 7q21.2. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane. Molecular Function: sterol 14-demethylase activity; iron ion binding; heme binding. Biological Process: xenobiotic metabolic process; cholesterol biosynthetic process; sterol metabolic process; steroid biosynthetic process; cholesterol biosynthetic process via 24,25-dihydrolanosterol

48-Strip-Wells

470 USD

96-Strip-Wells

675