ELISA/assay

Human Estrogen Receptor Alpha ELISA Kit

MBS721731

48-Strip-Wells

470 USD

ELISA Kit

Human Estrogen Receptor Alpha ELISA Kit

Estrogen Receptor Alpha

estrogen receptor alpha, partial; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

ERAlpha

ESR1; ESR; NR3A1; ER

ESR1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ER?. No significant cross-reactivity or interference between ER? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ER? and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ER? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ER?. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Princple of the assay: ER? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ER? antibody and an ER?-HRP conjugate. The assay sample and buffer are incubated together with ER?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ER? concentration since ER? from samples and ER?-HRP conjugate compete for the anti-ER? antibody binding site. Since the number of sites is limited, as more sites are occupied by ER? from the sample, fewer sites are left to bind ER?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ER? concentration in each sample is interpolated from this standard curve.

5821726

AAD52984.1

35,547 Da

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; integral to membrane; plasma membrane; nucleus. Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity. Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; gene expression; positive regulation of transcription factor activity; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating). Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance

48-Strip-Wells

470 USD

96-Strip-Wells

675