ELISA/assay

Rat Semaphorin-3A (SEMA3A) ELISA Kit

MBS7217298

48-Strip-Wells

470 USD

ELISA Kit

Rat Semaphorin-3A (SEMA3A) ELISA Kit

Semaphorin-3A (SEMA3A)

semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III

SEMA3A

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

SEM3A_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of SEMA3A. No significant cross-reactivity or interference between SEMA3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEMA3A and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This SEMA3A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SEMA3A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: SEMA3A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SEMA3A. Standards or samples are then added to the microtiter plate wells and SEMA3A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SEMA3A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SEMA3A are added to each well to "sandwich" the SEMA3A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SEMA3A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA3A concentration in each sample is interpolated from this standard curve.

5174673

NP_006071.1

NM_006080.2

88,889 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p12.1. Cellular Component: membrane; axon; dendrite; extracellular region. Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding. Biological Process: axon guidance; facial nerve structural organization; trigeminal nerve structural organization; apoptosis; regulation of heart rate; dendrite morphogenesis; olfactory bulb development; axonal fasciculation; neuron migration; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development. Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia

48-Strip-Wells

470 USD

96-Strip-Wells

675