ELISA/assay

Human Glucose Transporter 1 (GLUT1) ELISA Kit

MBS721715

48-Strip-Wells

470 USD

ELISA Kit

Human Glucose Transporter 1 (GLUT1) ELISA Kit

Glucose Transporter 1 (GLUT1)

Human Glucose Transporter 1 (GLUT1) ELISA Kit; Glucose Transporter 1 (GLUT1); Glucose Transporter 1 ELISA Kit (GLUT1) (Human)

glucose transporter 1, isoform G; CG1086 gene product from transcript CG1086-RG; glucose transporter 1, isoform G; CG1086-PG; Glut1-PG; lethal (3) S007412; glucose transporter 1, isoform D; CG1086-PD; Glut1-PD; glucose transporter 1, isoform F; CG1086-PF; Glut1-PF; glucose transporter 1, isoform E; CG1086-PE; Glut1-PE; glucose transporter 1, isoform B; CG1086-PB; Glut1-PB; glucose transporter 1, isoform C; CG1086-PC; Glut1-PC; Glucose transporter 1

GLUT1

Glut1; CG1086; DmelCG1086; Dmglut1; DmGLUT1; GLU; glut1; l(3)61Ea; l(3)L1H; l(3)S007412

Human

This assay has high sensitivity and excellent specificity for detection of GLUT-1. No significant cross-reactivity or interference between GLUT-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLUT-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Human ELISA Kit

Intended Uses: This GLUT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GLUT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay GLUT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLUT-1 antibody and an GLUT-1-HRP conjugate. The assay sample and buffer are incubated together with GLUT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLUT-1 concentration since GLUT-1 from samples and GLUT-1-HRP conjugate compete for the anti-GLUT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLUT-1 from the sample, fewer sites are left to bind GLUT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT-1 concentration in each sample is interpolated from this standard curve.

281365413

NP_728558.3

NM_167846.2

Developmental Biology Pathway (642261); Facilitative Na+-independent Glucose Transporters Pathway (642523); Glucose Transport Pathway (642308); Hexose Transport Pathway (642307); Integration Of Energy Metabolism Pathway (642408); Metabolism Pathway (642304); Metabolism Of Carbohydrates Pathway (642305); Metabolism Of Vitamins And Cofactors Pathway (642442); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (642443); Regulation Of Insulin Secretion Pathway (642409)

48-Strip-Wells

470 USD

96-Strip-Wells

675