ELISA/assay

Rat Cytochrome b-245 light chain (CYBA) ELISA Kit

MBS7217026

48-Strip-Wells

440 USD

ELISA Kit

Rat Cytochrome b-245 light chain (CYBA) ELISA Kit

[Cytochrome b-245 light chain (CYBA)]

[cytochrome b-245 light chain; Cytochrome b-245 light chain; cytochrome b-245 light chain; p22phox; cytochrome b light chain; p22 phagocyte B-cytochrome; cytochrome b(558) alpha chain; cytochrome b558 subunit alpha; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; flavocytochrome b-558 alpha polypeptide; neutrophil cytochrome b 22 kDa polypeptide; superoxide-generating NADPH oxidase light chain subunit; cytochrome b-245, alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox]

[CYBA]

[CYBA; CYBA; p22-PHOX; p22phox]

CY24A_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CXCR7. No significant cross-reactivity or interference between CXCR7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCR7 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This CXCR7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CXCR7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CXCR7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CXCR7 antibody and an CXCR7-HRP conjugate. The assay sample and buffer are incubated together with CXCR7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR7 concentration since CXCR7 from samples and CXCR7-HRP conjugate compete for the anti-CXCR7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR7 from the sample, fewer sites are left to bind CXCR7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR7 concentration in each sample is interpolated from this standard curve.

68509914

NP_000092.2

NM_000101.3

21,013 Da

Adaptive Immune System Pathway (366160); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway (477123); Disease Pathway (530764); Immune System Pathway (106386); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028); Leukocyte Transendothelial Migration Pathway (83083)

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family. Protein type: Oxidoreductase; Membrane protein, integral; EC 1.-.-.-. Chromosomal Location of Human Ortholog: 16q24. Cellular Component: Golgi apparatus; phagocytic vesicle membrane; cell soma; mitochondrion; membrane; apical plasma membrane; dendrite; plasma membrane; endosome; NADPH oxidase complex; secretory granule. Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding. Biological Process: response to drug; response to nutrient levels; respiratory burst; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; positive regulation of endothelial cell proliferation; smooth muscle hypertrophy; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process. Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415