catalog number :
MBS721695
products type :
ELISA Kit
products full name :
Human Lymphotoxin beta ELISA Kit
products short name :
Lymphotoxin beta
products name syn :
Human Lymphotoxin b ELISA Kit
other names :
Ltb; Ltb; lymphotoxin-beta; Ltb
products gene name syn :
LTB
other gene names :
LTB; Ltb
uniprot entry name :
A7X5T4_ORNAN
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This LyB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LyB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: LyB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LyB antibody and an LyB-HRP conjugate. The assay sample and buffer are incubated together with LyB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LyB concentration since LyB from samples and LyB-HRP conjugate compete for the anti-LyB antibody binding site. Since the number of sites is limited, as more sites are occupied by LyB from the sample, fewer sites are left to bind LyB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LyB concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ABU86912.1
ncbi mol weight :
25,099 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (88703); Cytokine-cytokine Receptor Interaction Pathway (460); NF-kappa B Signaling Pathway (634538); Rheumatoid Arthritis Pathway (200313); Rheumatoid Arthritis Pathway (200269)