ELISA/assay

Rat Matrix Metalloproteinase 8 ELISA Kit

MBS721664

96 Strip Wells

675 USD

ELISA Kit

Rat Matrix Metalloproteinase 8 ELISA Kit

Matrix Metalloproteinase 8

matrix metalloproteinase 8 (neutrophil collagenase); Neutrophil collagenase; neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase); matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL

MMP-8

MMP8; MMP8; HNC; CLG1; MMP-8; PMNL-CL; CLG1; MMP-8; PMNL-CL

MMP8_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This MMP-8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MMP-8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cancer

Principle of the Assay: MMP-8 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP-8. Standards or samples are then added to the microtiter plate wells and MMP-8 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for MMP-8 are added to each well to "sandwich" the MMP-8 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP-8 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-8 concentration in each sample is interpolated from this standard curve.

71648770

AAZ38714.1

53,412 Da

Activation Of Matrix Metalloproteinases Pathway 576264!!Collagen Degradation Pathway 730309!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262!!Matrix Metalloproteinases Pathway 198900

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD