ELISA/assay

Mouse Tissue Inhibitor of Metalloproteinases 1 ELISA Kit

MBS721646

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Mouse Tissue Inhibitor of Metalloproteinases 1 ELISA Kit

Tissue Inhibitor of Metalloproteinases 1

TIMP-1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); TIMP metallopeptidase inhibitor 1<; Embryogenin-1; EG-1; Tissue inhibitor of metalloproteinases 1; TIMP-1

TIMP-1

TIMP1; TIMP1; EG-1; TIMP-1

TIMP1_BOVIN

Mouse

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Cancer

INTENDED USE: This TIMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TIMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY: TIMP-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TIMP-1. Standards or samples are then added to the microtiter plate wells and TIMP-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TIMP-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TIMP-1 are added to each well to “sandwich” the TIMP-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TIMP-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-1 concentration in each sample is interpolated from this standard curve.

546974

AAB30892.1

23,032 Da

Activation Of Matrix Metalloproteinases Pathway (1008852); Degradation Of The Extracellular Matrix Pathway (1008851); Extracellular Matrix Organization Pathway (1008838); HIF-1 Signaling Pathway (695207); Hemostasis Pathway (1009046); Matrix Metalloproteinases Pathway (198565); Platelet Activation, Signaling And Aggregation Pathway (1009056); Platelet Degranulation Pathway (1009068); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1009066)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675