ELISA/assay

Rat Interleukin 16 ELISA Kit

MBS721637

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Interleukin 16 ELISA Kit

Interleukin 16

IL16 protein, partial; Pro-interleukin-16; pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor; interleukin 16; Lymphocyte chemoattractant factor; LCF

IL-16

IL16; IL16; LCF; NIL16; PRIL16; prIL-16; IL-16; LCF

IL16_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of IL-16. No significant cross-reactivity or interference between IL-16 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-16 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-16 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IL-16. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-16 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-16. Standards or samples are then added to the microtiter plate wells and IL-16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-16 are added to each well to "sandwich" the IL-16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-16 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-16 concentration in each sample is interpolated from this standard curve.

116283877

AAH50362.1

66,646 Da

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus. Molecular Function: cytokine activity. Biological Process: transcription, DNA-dependent; viral reproduction; regulation of transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675