ELISA/assay

Rat Arginase 2 (ARG2) ELISA Kit

MBS7216305

48-Strip-Wells

440 USD

ELISA Kit

Rat Arginase 2 (ARG2) ELISA Kit

[Arginase 2 (ARG2)]

[arginase 2; arginase 2; arginase type 2]

[ARG2]

[arg2]

Rat

This assay has high sensitivity and excellent specificity for detection of ARG1. No significant cross-reactivity or interference between ARG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARG1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This ARG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ARG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ARG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ARG1 antibody and an ARG1-HRP conjugate. The assay sample and buffer are incubated together with ARG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARG1 concentration since ARG1 from samples and ARG1-HRP conjugate compete for the anti-ARG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARG1 from the sample, fewer sites are left to bind ARG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARG1 concentration in each sample is interpolated from this standard curve.

638263160

NP_001279186.1

NM_001292257.1

Arginine And Proline Metabolism Pathway (996399); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (996461); Biosynthesis Of Amino Acids Pathway (795174); Metabolic Pathways (996456); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (998675); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (468321); Urea Cycle Pathway (998635); Urea Cycle Pathway (468222)

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415