catalog number :
MBS721595
products type :
ELISA Kit
products full name :
Human Survivin ELISA Kit
products short name :
Survivin
other names :
survivin; survivin
products gene name :
Surv
other gene names :
LOC398389
specificity :
This assay has high sensitivity and excellent specificity for detection of Survivin. No significant cross-reactivity or interference between Survivin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Survivin and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.
products categories :
Cell Biology
products description :
Intended Uses: This Survivin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Survivin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay Survivin ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Survivin antibody and an Survivin-HRP conjugate. The assay sample and buffer are incubated together with Survivin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Survivin concentration since Survivin from samples and Survivin-HRP conjugate compete for the anti-Survivin antibody binding site. Since the number of sites is limited, as more sites are occupied by Survivin from the sample, fewer sites are left to bind Survivin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Survivin concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001082294.1
ncbi gb acc num :
NM_001088825.1
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)