ELISA/assay

Mouse Amphiregulin ELISA Kit

MBS721572

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Mouse Amphiregulin ELISA Kit

Amphiregulin

amphiregulin; Amphiregulin; amphiregulin; amphiregulin B; schwannoma-derived growth factor; colorectum cell-derived growth factor; amphiregulin; Colorectum cell-derived growth factor; CRDGF

AREG

AREG; AREG; AR; SDGF; AREGB; CRDGF; SDGF AREGB ; AR; CRDGF

AREG_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of AREG. No significant cross-reactivity or interference between AREG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AREG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This AREG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AREG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Princple of the assay: AREG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AREG. Standards or samples are then added to the microtiter plate wells and AREG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AREG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AREG are added to each well to “sandwich” the AREG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AREG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AREG concentration in each sample is interpolated from this standard curve.

179040

AAA51781.1

27,895 Da

ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); FSH Signaling Pathway (672455); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus. Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; G-protein coupled receptor protein signaling pathway; cell proliferation; response to hydrogen peroxide; cell-cell signaling; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of phosphorylation; positive regulation of DNA replication

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675