ELISA/assay

Human beta 3 adrenergic receptor (ADRB3) ELISA Kit

MBS7215697

48-Strip-Wells

470 USD

ELISA Kit

Human beta 3 adrenergic receptor (ADRB3) ELISA Kit

beta 3 adrenergic receptor (ADRB3)

Human b 3 adrenergic receptor (ADRB3) ELISA Kit

Beta 3 adrenergic receptor; Beta-3 adrenergic receptor; beta-3 adrenergic receptor; beta-3 adrenoceptor; beta-3 adrenoreceptor; adrenergic, beta-3-, receptor; adrenoceptor beta 3; Beta-3 adrenoreceptor; Beta-3 adrenoceptor

ADRB3; ADRB3; BETA3AR; ADRB3R; B3AR; Beta-3 adrenoceptor

ADRB3_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ADRbeta3. No significant cross-reactivity or interference between ADRbeta3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRbeta3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This ADRbeta3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADRbeta3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

. Principle of the assay: ADRbeta3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADRbeta3 antibody and an ADRbeta3-HRP conjugate. The assay sample and buffer are incubated together with ADRbeta3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRbeta3 concentration since ADRbeta3 from samples and ADRbeta3-HRP conjugate compete for the anti-ADRbeta3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRbeta3 from the sample, fewer sites are left to bind ADRbeta3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRbeta3 concentration in each sample is interpolated from this standard curve.

312397

CAA50141.1

P13945

43,519 Da

Adrenoceptors Pathway (106365); Amine Ligand-binding Receptors Pathway (106363); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Endocytosis Pathway (102279); Endocytosis Pathway (102181); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548)

The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq, Feb 2009]

ADRB3: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: integral to plasma membrane; plasma membrane; nucleus; receptor complex. Molecular Function: beta3-adrenergic receptor activity; protein binding; protein homodimerization activity; beta-3 adrenergic receptor binding; norepinephrine binding; epinephrine binding; beta-adrenergic receptor activity. Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; diet induced thermogenesis; generation of precursor metabolites and energy; negative regulation of multicellular organism growth; eating behavior; adenylate cyclase activation; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; response to antibiotic; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of MAPKKK cascade; heat generation; carbohydrate metabolic process; brown fat cell differentiation; energy reserve metabolic process; response to cold; aging; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure. Disease: Obesity

48-Strip-Wells

470 USD

96-Strip-Wells

675