ELISA/assay

Rat Aggrecan ELISA Kit

MBS721531

48-Strip-Wells

470 USD

ELISA Kit

Rat Aggrecan ELISA Kit

Aggrecan

aggrecan core protein; Aggrecan core protein; aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage; aggrecan; Cartilage-specific proteoglycan core protein; CSPCP

AGC

Acan; Acan; Agc; Agc1; Agc; Agc1; CSPCP

PGCA_RAT

Rat

This assay has high sensitivity and excellent specificity for detection of AGC. No significant cross-reactivity or interference between AGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Signal Transduction

Intended Uses This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: AGC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGC. Standards or samples are then added to the microtiter plate wells and AGC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGC are added to each well to "sandwich” the AGC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.

11990616

NP_071526.1

NM_022190.1

225,171 Da

Endochondral Ossification Pathway (219771); Spinal Cord Injury Pathway (739006)

an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675